I want to check uptake of my dye loaded particles into cells. I face challenges during preparation step of particles . Particle size and polydispersity is so high when i disperse particles in water for size analysis. The dye because of its planar nature, hydrophobicity forms aggregates in water interfering with actual size of particles.
Rhodamine B: This dye is less hydrophobic than Nile Red.
Coumarin 6: Another hydrophobic dye, but it has been used successfully in various nanoparticle formulations. It might offer better stability in your system.
Fluorescein: This dye is hydrophilic and might not aggregate as much in water. However, its fluorescence properties differ from Nile Red, so ensure it fits your detection system.
Adding a surfactant can help stabilize and prevent aggregation. Additionally, employing the sonication method, which uses ultrasonic waves, can assist in breaking down aggregates to achieve a more uniform particle size distribution.
Regards and Good luck
Adnan Shahzaib thanks for the suggestions, well i cannot go with hydrophilic dyes as i want to mimic kinetics with some hydropohobic dye. Adding surfactant worked in my case.
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