I found in this website https://answers.yahoo.com/question/index?qid=20080313122043AA3Ls3F that the dimension of a nucleotide is 0.34 nm. Is there any experimental paper confirming this statement? Tx
Hello, I did the PCR (long-range, target ~5 Kb) that is shown in the attached file. The lanes are: 1. ladder 2. target (plasmid purified from E. coli after transfection), 50 ng/uL corresponding to...
01 February 2020 3,475 3 View
Hello, from this website (http://phage.org/names/2000/) it is possible to determine the genera targeted by a family of bacteriophages, for instance the phage species DS96 , H24 , M35 , P3 , P9 ,...
06 July 2019 7,028 7 View
I am trying generate a custom general feature format (GFF) file but I need to calculate the phase of the Coding Sequence (CDS) for split ORFs, that is ORFs that have multiple CDS; for instance I...
09 October 2018 2,983 0 View
Hello, I am using Linux and I tried to index the human genome built 38 (GRCh38). I have available 62.9 GiB of RAM. I ran the command: `bwa index -a bwtsw ` In theory, the process should use up...
07 August 2018 6,793 0 View
Hello, is there a journal that publishes computer code for bioinformatics purposes but it is not so much high end (no Oxford or Nature class to be clear). The code I have written is not mind...
02 March 2018 3,801 0 View
Dear all, I am working at using ligation-mediated PCR (LM-PCR) to amplify DNA of unknown origin; at the moment I am testing the system using amplicons, which should have the terminal adenine at...
04 May 2017 8,725 3 View
Is there a good paper (or set of them) that I can follow to properly and quickly set up a ligation mediated PCR (LM-PCR)? Are there tips for the design of the adaptors? How to melt together the...
03 April 2017 406 14 View
It is now customary from the journal side to ask the authors for an upfront fee in order to enable free accessibility to the article. The fee is in the neighbour of 1000 pounds and many...
10 November 2016 8,581 3 View
I'd like to know the features of single stranded RNA molecules such as diameter and charge, but I can't find any experiment on the subject. There are some works on dsRNA and hairpins, but nothing...
06 July 2016 9,458 2 View
It is common knowledge that the accepted range for the efficiencies of a qPCR is between 90 and 110 percent whereas the Y-intercept should be between 33 and 37 cycles (data coming from Life tech...
11 December 2015 9,693 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Is this website real? https://isar.org.in/event/registration.php?id=2434532
08 August 2024 484 1 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
how to get links for copyrights for papers?
06 August 2024 7,410 1 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View