16 Questions 22 Answers 0 Followers
Questions related from Luigi Marongiu
Hello, I did the PCR (long-range, target ~5 Kb) that is shown in the attached file. The lanes are: 1. ladder 2. target (plasmid purified from E. coli after transfection), 50 ng/uL corresponding to...
02 February 2020 3,453 3 View
Hello, from this website (http://phage.org/names/2000/) it is possible to determine the genera targeted by a family of bacteriophages, for instance the phage species DS96 , H24 , M35 , P3 , P9 ,...
07 July 2019 6,994 7 View
I am trying generate a custom general feature format (GFF) file but I need to calculate the phase of the Coding Sequence (CDS) for split ORFs, that is ORFs that have multiple CDS; for instance I...
10 October 2018 2,934 0 View
Hello, I am using Linux and I tried to index the human genome built 38 (GRCh38). I have available 62.9 GiB of RAM. I ran the command: `bwa index -a bwtsw ` In theory, the process should use up...
08 August 2018 6,756 0 View
Hello, is there a journal that publishes computer code for bioinformatics purposes but it is not so much high end (no Oxford or Nature class to be clear). The code I have written is not mind...
03 March 2018 3,769 0 View
Dear all, I am working at using ligation-mediated PCR (LM-PCR) to amplify DNA of unknown origin; at the moment I am testing the system using amplicons, which should have the terminal adenine at...
05 May 2017 8,694 3 View
Is there a good paper (or set of them) that I can follow to properly and quickly set up a ligation mediated PCR (LM-PCR)? Are there tips for the design of the adaptors? How to melt together the...
04 April 2017 374 14 View
It is now customary from the journal side to ask the authors for an upfront fee in order to enable free accessibility to the article. The fee is in the neighbour of 1000 pounds and many...
11 November 2016 8,553 3 View
I found in this website https://answers.yahoo.com/question/index?qid=20080313122043AA3Ls3F that the dimension of a nucleotide is 0.34 nm. Is there any experimental paper confirming this statement? Tx
07 July 2016 9,887 0 View
I'd like to know the features of single stranded RNA molecules such as diameter and charge, but I can't find any experiment on the subject. There are some works on dsRNA and hairpins, but nothing...
07 July 2016 9,435 2 View
It is common knowledge that the accepted range for the efficiencies of a qPCR is between 90 and 110 percent whereas the Y-intercept should be between 33 and 37 cycles (data coming from Life tech...
12 December 2015 9,664 4 View
Dear all, I would like to ask what is the accepted rate of agreement between different operators in rating the results from (quantitative) PCR. Assuming I have a set of PCR results and different...
11 November 2015 3,812 0 View
Dear all, I am trying to implement a method to read qPCR runs using the qpcR package developed by Andrej-Nikolai Spiess. I am almost there, but when I try to apply the cy0() function to my actual...
03 March 2015 8,506 3 View
I am trying to set a model using the pcrfit() function of the R's qpcRpackage. I have defined a dataframe with 45 cycles (rows) and 4readings (columns) derived from a TaqMan qPCR with...
03 March 2015 4,845 3 View
Hello, is there a company that purchase phages just like ATCC does for microbes (they have some phages but few) and cell lines? Alternatively, is there a good method for isolating phages against a...
01 January 1970 4,920 4 View
single chain variable fragments (scFv) antibodies can be expressed either in soluble form or on the surface of bacteriophages (phage displayed); in both cases, they are produced essentially using...
01 January 1970 6,204 0 View
