Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

Why Negative peak eluted in HPLC analysis?

I'facing problem while doing HPLC analysis due to negative peak was eluted which was corresponding to the principle peak RT. I tried to remove, with different solvents, but no use. i want to know...

26 November 2020 1,960 5 View

Is 2 mm thickness carbon fiber plate is sustainable at high pressure high speed gas impact and how?

Interested in Reaserch to join steel material with carbon fiber for better stiffness and mechanical properties.

19 November 2020 9,790 2 View

What does variation in FTIR peak width after doping signify?

FTIR peaks are related to bond vibrations. In that context, what does the variation in the peak width after doping signify? Is it because of the variation in bond length or any kind of microstrain...

13 July 2020 6,009 1 View

Is there any list available of GRAS (generally recognized as safe) microorganisms with proper references?

Is there any list available of GRAS (generally recognized as safe) microorganisms with proper references?

02 March 2020 1,011 2 View

Are we good with non-stationarity of financial data in OLS regression?

Monthly data of oil prices is one of my independent variables. The purpose of doing this analysis is to get the relationship of oil prices with other financial variables (bond spreads, equity...

21 February 2020 6,978 1 View

Natural farming can fuilfil food requirements of a farm family?

Natural Farming is a concept parallaly emerge with organic Farming. Wheather it can produce sufficient food to an individual farm family.

05 October 2019 9,543 3 View

Is there is any toxic secreation in the initial phase of addtion of organic material like plant leaves etc?

after applicationof organic material is there is toxic chemical found in the field that may cause or devlop toxic or laethal chemical.

27 September 2019 9,227 5 View

Which stain is used to see the deposition of oil or oil glands in anatomy section of leaf(transverse section) in cymbopogon?

Cymbopogon is an aromatic grass which produces essential oils as a secondary metabolite so to see the deposition of oil in leaves transverse section of intra and inter species can you suggest me...

28 August 2019 9,357 2 View

What is the difference between lithium ion and lithium polymer battery?

Lithium ion and lithium polymer battery charging and discharging profile was found to be same. Then what is the major difference between both battery?

07 July 2019 9,301 4 View

No title

02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View