I have seen in papers that many papers remove singletons from their microbiota data. I am not sure what is actually singletons and why the authors remove the singletons sequences. I would be happy if you share your thoughts and experiences about this matter. Thank you in advance for helping me.
To simply put, a singleton means a sequence that cannot be matched to its counter strand.
Examples)
5'-ATGCAATTGAA-3'
3'-TACGTTAACTT-5'
These aligned pairs are correctly sequenced.
5'-ATGCAATTGAA-3'
3'-TNNANNAANN-5'
In this example, only one strand is sequenced, and the counter pair cannot be matched, so the sequence is a singleton.
Please refer to following pages:
https://drive5.com/usearch/manual/singletons.html
http://seqanswers.com/forums/showthread.php?t=46711
A singleton is a OTU (Operational Taxonomical Unit) with only one sequence. They are removed to reduce sequencing errors.
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