I am looking for a standardized method for detection of interferon alpha 2b protein in bulk serum concentrate and compare with a standard interferon used for medicine production. What are the techniques to detect any change in protein?
Multiplex PCR can be used to detect hIFN alpha 2b gene. You can get by go through the following link.
http://www.ncbi.nlm.nih.gov/pubmed/23074956
Try VeriKineTMHuman IFN-a ELISA Kit (Product # 41100-1) from PBL Assay Science. It worked very well for me
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