After Electrospinning of PCL Nanofibers and Aminolysis via Ethylendiamine treatment and N2 Plasma treatment we have cultured Smooth muscle cells on these scaffold. in SEM images below we see fractures between cell-nanofiber interfaces. we have done the same with HUVECs and nothing like these fractures were observed.
I have experienced similar situations particularly after fixing the cells with glutaraladehyde for SEM analysis. Given the fixation treatment and freeze drying conditions, it seems that the cells shrink due to dehydration upon such treatment and most likely stretch the fibers along with it which leads to fracture.
I strongly think that it is an artifact due to fixation, such as stated by Ashang. I will try less strong fixation conditions. Let me know.
Try a different fixation protocol, but that might not be the reason. Some times contractile cellular forces can detach surface nanostructures (Depending on the cell type). I have had similar experiences with BMSCs. Good luck
Hi, what you observed is due to the washing processed during the fixation try using a more biological fixation process methanol 2 min -20°c and then heat-freeze your simple.
Best.
Philippe
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