What is PC3 Cell culture condition?

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Kar Wei Chin

around 30-40% yesterday and they all detached today. May I also what is the doubling time, is it and 33hrs? Areya Tabatabai

Areya Tabatabai

Kar Wei Chin the doubling time is usually between 24-36 hours for PC3 cells.

Regarding the floating cells, are you adding enough media to the flask to stop the activity of the trypsin? i.e. if you are using 3mL of trypsin I would add 9mL of media.