I´m no specialist for your problem, but here is an append showing an empirical method for path length correction in microplate reader. Perhaps it can help.

I´m no specialist for your problem, but here is an append showing an empirical method for path length correction in microplate reader. Perhaps it can help.

Thank you Mr. Hanno

If you are using a cuvette, the pathlength is defined by the construction of the cuvette, typically 1-cm. Pathlength correction for plate readers applies to microplates, in which the pathlength is the height of the liquid in the well.