What is other assay besides vanillin-sulfuric acid assay to test for saponin?

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Muhammad Mushtaq

You should try to quantify extracted saponins by HPLC

Afrina Zainal

Thank you for your suggestion Mr. Muhammad Mushtaq.

Frank Höger

Hi Afrina

if you want to try another TLC visualization you have this possiblilities

1. Komarowskys Reagent

4-Hydroxi-benzaldehyde (2%) in Methanol and 50% sulfuric acid (mixed)

2. one of the Cer(V)sulfat reagents for alkaloids

some saponines also have a chance to be analyzed in GC