What is other assay besides vanillin-sulfuric acid assay to test for saponin?

More Afrina Zainal's questions See All

What is the wavelength to measure OD of Sf9 cell culture?

Hi. Does anyone know what is the wavelength for Sf9 cell culture? Is the wavelength different if i use spectrophotometer or microplate reader? Thanks.

09 October 2017 1,260 3 View

How to search for possible compound based on peak absorbance?

Hi. I constructed an absorption spectrum of my plant extract, ranging 200-700 nm, using UV-vis spectrophotometer. I obtained four peaks. How to search for possible compounds that represents the...

09 October 2017 7,360 5 View

What is the principle of vanillin-sulfuric acid assay to quantify saponin?

I'm doing extraction of saponin from banana peels. i use vanillin-sulfuric acid assay to but got some error. all my samples become black after incubation and give 3.00 absorbance. may i know what...

08 September 2017 4,273 6 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Muhammad Mushtaq

You should try to quantify extracted saponins by HPLC

Afrina Zainal

Thank you for your suggestion Mr. Muhammad Mushtaq.

Frank Höger

Hi Afrina

if you want to try another TLC visualization you have this possiblilities

1. Komarowskys Reagent

4-Hydroxi-benzaldehyde (2%) in Methanol and 50% sulfuric acid (mixed)

2. one of the Cer(V)sulfat reagents for alkaloids

some saponines also have a chance to be analyzed in GC