How can I confirm that drug loaded into formulat is in amorphous or crystalline form without carrying out XRD,knowing that that DSC & FTIR are done
XRD analysis is a direct method for determining the ratio of crystalline and amorphous phases. DSC shows phase transitions, and can fix the transition from an amorphous phase to a crystalline one (devitrification) and melting, and can also fix the evaporation of moisture. There is a method for determining crystallinity using DSC. https://www.researchgate.net/post/How-do-I-calculate-crystallinity-using-a-DSC-curve/1 FTIR shows the amorphous and crystalline phases, their spectra have differences (peak shift, there may be wider lines for the amorphous phase), but the phase ratio cannot be calculated. It should also be noted that in order to accurately calibrate the phase ratio calculation, samples may only be required in the amorphous phase and only in the crystalline phase.
Dear Dr. Abeer Osama Motawee
For amorphous and crystalline characteristics, the FTIR and DSC diagnoses were enough.
Best regards...
Dear Abeer Osama Motawee . Don't know about IR, but with DSC you can see if there is an amorphous phase in your system. Attached is an paper that describes the main points of this analysis (Paper 1). In addition, I propose to consider the option of using a microscope with polarized light. The amorphous form of a component under a polarized microscope will not appear in color, just clear so that the color matches the observation base in the microscope. This experiment does not require special equipment, only a microscope with an appropriate device. I am also attaching an paper where a similar experiment was carried out (Paper 2).
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