For secretion of recombinant protein in pichia pastoris, the most useful secretion signal is alpha-factor secretion signal from S. cerevisiae .
In some vectors( e.g. pPICZalpha A, Invitrogen) this secretion signal is in complete form (85aa). but in some cases, just first 19aa(presequence) is used for secretion of recombinant protein.
what are differences between these truncated and complete form of secretion signal?is there any advantage to use truncated form?
The alpha-factor secretion signal is often not cleaved completely and can lead to n-terminal heterogeneity, see: https://www.bioc.uzh.ch/plueckthun/pdf/APpub0330.pdf
According to the following publication (where the signal peptide was changed), the sequence following the signal peptide (which is 66aa long) improves packaging for exportation through COPII vesicles.
Article An improved secretion signal enhances the secretion of model...
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