What is difference between plastic stain and total percentage of elogation?, how can calcultate plastic strain?

01 November 2023 0 5K Report

Badges
Science topic

Similar topics
Academic Journals

More Uttam kumar Pandit's questions See All

Root-AMF colonization analysis non-destructive method?

Is there any non-destructive method (not to harvest the root) to quantify AMF colonization with plant roots in pot experiments or rhizotrons?

02 October 2023 9,449 0 View

I am working on graphene based supercapacitor I wanted to know how to make a comparison between the estimated capacitance of CV and GCD?

Please suggest me as they differ in their measurement principle

11 April 2023 2,775 1 View

Can anyone suggest where would I get the codes(C++ or any) for charge exchange cross-section of positive to negative ion converting atomic reactions?

The codes to plot the cross-section vs energy curve and also the research article that might help in understanding, theoretically and mathematically, the reactions of the form: A^+ + B^-...

15 January 2023 8,925 2 View

How to calculate Palmer Drought Severity Index and scPDSI on R, if we have only station climate data of Temperature and Precipitation?

I have only Temperature and precipitation data of climate station

13 December 2022 1,204 4 View

How I can calculate saturation, 75% and 50% of saturation in pot experiment of rice?

For setting an pot experiment

02 November 2022 2,668 3 View

How do you determine what the compound actually is based on it's m/z ratio?

I did a metabolomic study, where some of my compounds are novel and have high production in one of my mutants. I have its m/z ratio. Can I identify this compound solely based on this ratio? I...

06 October 2022 8,248 4 View

What is the basic difference between time-correlated single photon counting (TCSPC) and Fluorescence Lifetime Imaging microscopy (FLIM)?

Is there any difference between time-correlated single photon counting (TCSPC) and Fluorescence Lifetime Imaging microscopy (FLIM)? TCSPC i.e. lifetime measurement of the fluorophore is basically...

01 September 2022 5,780 6 View

Do I have to do RNAcleanup after DNAseI treatment and heat inactivation of DNAse?

I am extracting RNAs using Trizol combined with Purelink mini kit and I am doing post isolation DNAse treatment for making RNA free from genomic DNA. After DNAse treatment I do inactivate DNAse...

02 November 2021 7,086 2 View

Hi there! Do anyone has experience in terms of efficiency of on column DNAse treatment vs post extraction DNAse treatment during RNA extraction?

I am doing RNA extraction from pea plant roots for sequencing purpose and require good quality RNA without contamination. I am using Purelink RNA Mini Kit for extraction. I found on column DNAse...

28 October 2021 2,344 0 View

How to plot force vs time plot and force-extension plot from Steered MD Simulation data?

I have done steered MD of RNA and now trying to figure out the analysis of the data obtained by the simulation.. I wanted to know on how to plot the force vs time plot and force-extension plot...

26 October 2021 4,964 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Water Consumption in Plastic Recycling: Is it Higher Than for Virgin Plastics?

Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...

29 July 2024 4,630 0 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View