Dear Mohamed,

to obtain PRP you can centrifuge blood sample at 180gx15-20' and importantly use  NO brake settings at the centrifuge. To have PRP sterile I suggest to open blood tubes under a tissue culture hood.

Greetz

Claudia

Hi,

I use 100 x g for 10 minutes without any brakes in the centrifuge... just take commercial citrated tubes for blood collection and work under a sterile bench to have sterile conditions....

Greetz

Kai

Dear Mohamed, we use 200 x g for 15 min, NO brake and room temperature (never cold) at the centrifuge. We open the tubes under tissue culture hood to have sterile conditions. 

Many thanks for all of your contributions , But my questions about available commercial closed mehods

I do not know if this is of help: Why not buy plateletapheresis concentrates? These are nothing else than PRP, collected in a sterile, closed system!

What do you need the PRP for?

autologous PRP  can be used in many clinical settings to enhance wound healing

julian Strobel

Ah, ok, so it is a clinical setting. We have done autologous PRP (even very highly concentrated) with the TRIMA Accel for such reasons, but only in very small numbers. However, this works fine! Problem: You need expensive equipment! And your patient needs to be fit enough for an extra corporal circulation at the cell separator.

Many thanks,  julian,

An other system, like that  product of Harvest is good & can overcome the problem of  extra corporal circulation, But I think also expensive.

300 g for 12 min at 120C