I am working with biosurfactant producing bacteria. I have isolated and characterized a number of biosurfactant producing bacteria. I want to preserve these bacteria for future work. So I need to lyophilization this culture.
Simply dd 15% sterile glycerol to bacterial culture. Vortex the culture to ensure that glycerol is evenly dispersed and immediately frozen to –80°C for long-term storage. In theory, bacterial cultures containing glycerol can be stored indefinitely. As suggested by Vincent, to recover the bacteria, don’t thaw the vial but simply scrape the frozen surface of the culture and seed in plate or medium containing the appropriate antibiotic.
Why won't you use the glycerol preservation in -80 Celcius degrees? It preserves living bacteria for years and allow you to work on the same culture... Usually we use 50% Glycerol ( in DDW) and add it 1:1 with liquid culture. Mix and store in -80 degrees.
As for liophilization, you need to freeze the bacteria -80 or liquid nitrogen (better) and enter the frozen samples to liophilizer untill it is compleatly dry. Store in -80 Celcius degrees. However I am not sure you will be able to recover living baceria. Even if you do , you will be able to do it only once...
I would try the glycerol stocks first.
Good luck :)
Add 15% sterile glycerol to the culture. Vortex the culture to ensure that glycerol is evenly dispersed in the culture and immediately frozen to –80°C in deep freezer for long-term storage.
Realizar una disolución al 10% de leche en polvo desnatada en agua destilada, añadir 5-10% de glicerol, esterilizar (filtración, autoclave, tindalización...); alicuotar según interese y ya tienes el medio adecuado para suspender tus bacterias en él congelando a < de -20ºC.
For bacterial culture go for liquid nitrogen. In lyophilization u can prserve in vial and one should go for heating above 100 degree.
If you want to use bacteria frequently then best way is prepare slants in a tube and keep at 4 dc so this bacterial culture will be active for 3 to 4 months and after that again subculture the same other is glycerol Stock and stab
Do glycerol stock cultures act well for storage of fungal cultures or is their any other suggested method for lyophilization of fungal strains?
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