Say for example is 50/50 an acceptable value? After what number should we stop aiming for higher values? Below what percentage of live cells should we abandon the experiemtnas erroneous or unacceptable?
Stain the cells with Zombie UV fixable viable stain to differentiate between viable/non viable cells. Since flow cytometric analysis is primarily focused on proportions instead of absolute numbers, a 50% viability should be fine.