Can anyone suggest me a suitable vehicle to dissolve a drug that is already dissolved in DMSO to be administered in vivo to mice? This drug seems to be insoluble in saline or PBS (it precipitates) and appears cloudy when dissolved in water. I was suggested to use cyclodextrin but we don't have a stock of it right now. Is there any other alternative than cyclodextrin to dissolve this kind of drug?
We thought of administering the drug with DMSO completely, but there is a legal limit to the amount of it to be given to animals.
If you can make fine suspension of your drug in Tween 80 (0.5%) it will be easy for you.
u can use peanut oil to dissolve the substance (5% DMSO in peanut oil; disssolve ur substance in DMSO and add peanut oil to that to make it 5% solution). u can give it by intraperitoneal route for frequent administration. For one or two time administration, u can choose oral route. But if ur administration is for more than two times its better to use intraperitoneal route. Because more than two times administration by oral route can cause laxative effect (as it contains peanut oil) and drug may be partially absorbed.
if you will administer such preparation by intraperitoneal route it will damage the cavity and animal will die within 8 days. If you will give by subcutaneous route then it will form depot and act as sustained release.
@nikhil sakle.. Your response is poor.. I think you donot work much on lab animals..
Please refer this link..
Http://www.ncbi.nlm.nih.gov/pubmed/21983441
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