Anusha Jayaraman

3 Questions 4 Answers 0 Followers

Questions related from Anusha Jayaraman

Is this an effect of pH of the culture media (medium prepared in lab)?

I prepared a custom media with almost all essential components added individually (glucose, glutamine, all other amino acids, sodium pyruvate, sodium bicarbonate and HEPES), for an experiment. I...

30 March 2019 8,050 0 View

Rod to spore shaped 'particles' in cell cultures, bacterial contamination?

Last week I had consecutive contaminations of some of my mammalian primary cells alone. It started with the cultures turning turbid after a day or two of incubation. I wiped down the entire...

12 February 2019 5,269 8 View

Is there a best method for processing cell culture media samples for GC/MS? In specific for tryptophan/kyn pathway metabolites?

This is for pre-processing, before the derivatization step. I used trichloroacetic acid 50%, but after spinning down I seem to lose some significant amount of media sample and the result does not...

30 July 2018 6,248 3 View