Providon-iodine is the industrial solution containing an active compound iodide. The question is what is a suitable concentration can be used to kill pathogen.
Dear Mohemid, you can try both MIC and Killing assay to find out the minimum concentration which is required to inhibit and will be able to kill the pathogens. This can be done in different formats using either broth cultures of the pathogen or plating methods. For this you can try serial dilution of the test compound and test with your pathogen. You may try to google various protocols of MIC and killing assays. All the best.
I'm not sure which pathogens you are interested in, but I've had a look in our database and we have a page on Povidone-iodine including minimum inhibitory concentrations for several Candida species. You can see the page here: https://www.researchant.org/compound/2637.html
Why you are strict on Iodine? Can I suggest any other contain Chlorine and/or bromine which are use beautifully for killing pathogen. If you are interested . Pl send me the type of target microbes you want to kill.
Dear Satadal,I think bromine is a health risky chemical to be used in such routine practices even sometimes prhibited to be used as one of side effects is infertility and more.....