Carotenoids are widely known as provitamin A, while there is an increasing interest in their role as antioxidants. For this purpose carrot roots are cut to slices (width 2 mm, length 1 cm). The extraction yield of carotenes is observed at different temperatures (20°C, 40°C, and 60°C) using ethanol and 2-propanol. Initially, 25 g of cut carrot samples are added to 100 g of 96% ethanol. Carrot slices are extracted in water bath (20°C, 40°C, 60°C), shake after every 10 min, and after every hour of extraction 5 ml sample are s taken and mixed with petroleum ether (20 ml). Water is
added for the separation of phases, and after the separation the petroleum-ether-carotenoid phase is made up to the volume of 50 ml.
Take carrot extract carotene from carrot roots , crush well in a mixture and extract in ethanol & filter to remove solid mass then concentrate the solution.. Thick mass extracted in pet. ether & conentrated by rotary evaporation to get orange coloured carotene in the flask
Carotene can be obtained from carrot roots by water or alcoholic extraction or extraction with petroleum ether. Also follow the method suggested by Pandey (1980 & 1990) or according to Mr. M.B. Deshmukh.
Not with water, carotenoids are not water-soluble. If you want something like a reasonable yield, you will need fairly apolar solvents. Acetone, various alcohols (methanol, ethanol, propanols), ethylacetate, hexane, supercritical CO2 are used industrially. It is easy to check, as long as the carrot mass is orange your extraction is not complete.
The better way for me is extraction with acetone followed by partition with petroleum ether. You will obtain a clear and water free extract.
carotene can not be extracted with just ethanol because ethanol is going to remove water content of carrot and will remain oil a lot of oil. i think that oil could be removed be adding chloroform and then decantation the solution.
Use a combination of methanol and dichloromethan, and then further proceed.
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