What is a nucleus-specific marker for mouse RGCs?

01 January 2019 0 2K Report

I would like to try single nuclei RNASeq of mouse RGCs, and I was thinking of Rbpms. It needs to be specific for both RGCs and their nuclei. Any ideas?

Badges
Science topic

Similar topics
Cognitive Science
Thinking

More Jane Lindborg's questions See All

What's the best way to confirm raised antibody levels in mouse serum after injecting the same antibody?

I just want to know that after in vivo antibody injection, the levels of antibody are higher several hours post-injection compared to several days post-injection. I'm not sure if antibody...

06 July 2019 7,233 3 View

Where can I get a Cas9 virus of known viral titer to use for a standard curve for qPCR?

I've made a Cas9 virus using the #60957 plasmid from Addgene, and I need to titer it using qPCR. There's no GFP reporter, and I'd prefer not to target the AAV ITRs because there are two of them...

04 May 2018 6,973 3 View

Does anyone have a protocol for the in vivo injection of annexin v in mice to potentially block phagocytosis?

I'm also looking for a company that sells annexin v for this purpose.

02 March 2015 3,615 1 View

What is the best way to extract RNA from mouse sciatic nerves?

I've been using trizol to homogenize frozen nerves. I've pooled 2, 4, and 6 nerves and ran qPCR for GAPDH. The Ct values for all combinations were about the same (~23). So I used 2 nerves to run a...

02 March 2015 7,399 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View