Jane Lindborg

5 Questions 5 Answers 0 Followers

Questions related from Jane Lindborg

What's the best way to confirm raised antibody levels in mouse serum after injecting the same antibody?

I just want to know that after in vivo antibody injection, the levels of antibody are higher several hours post-injection compared to several days post-injection. I'm not sure if antibody...

07 July 2019 7,256 3 View

What is a nucleus-specific marker for mouse RGCs?

I would like to try single nuclei RNASeq of mouse RGCs, and I was thinking of Rbpms. It needs to be specific for both RGCs and their nuclei. Any ideas?

01 January 2019 2,003 0 View

Where can I get a Cas9 virus of known viral titer to use for a standard curve for qPCR?

I've made a Cas9 virus using the #60957 plasmid from Addgene, and I need to titer it using qPCR. There's no GFP reporter, and I'd prefer not to target the AAV ITRs because there are two of them...

05 May 2018 6,996 3 View

What is the best way to extract RNA from mouse sciatic nerves?

I've been using trizol to homogenize frozen nerves. I've pooled 2, 4, and 6 nerves and ran qPCR for GAPDH. The Ct values for all combinations were about the same (~23). So I used 2 nerves to run a...

03 March 2015 7,437 3 View

Does anyone have a protocol for the in vivo injection of annexin v in mice to potentially block phagocytosis?

I'm also looking for a company that sells annexin v for this purpose.

03 March 2015 3,648 1 View