I am measuring common carp metabolism using a respirometry box. Regarding that, I have measured routine metabolic rate (RMR) and maximum metabolic rate (MMR) in rest and unfed fish, and following exhaustive activity respectively.
Now, I am going to calculate aerobic scope (or metabolic scope) that seems to calculate SMR initially. The question is how does calculate the SMR without measuring fish activity (swimming speed)?
I suggest that you make a frequency distribution of all your resting/routine determination and the fit them to the sum of two normal distributions - as described in "Steffensen, J. F., Bushnell, P. G. & Schurmann, H. (1994). Metabolic rate of 4 Arctic species of teleosts from Greenland. Polar Biology, 14; 49-54" that can be downloaded from here: http://www.mbl.ku.dk/jfsteffensen/Publications/PolarBiol%201993%20JFS.pdf or from Research Gate.
The normal distribution to the left represent resting or standard metabolic rate - the normal distribution to the left routine metabolism.
This method requires that you have quite a few metabolic rate determinations. This can be obtained by intermittent respirometry as described in: " Steffensen, J.F. (1989). Some errors in respirometry of aquatic breathers: how to avoid and correct for them. Fish. Physiol. Biochem. 6; 49-59" that can be downloaded from here"http://www.mbl.ku.dk/jfsteffensen/Publications/FishPhysiolBiochem%201989%20JFS.pdf or from Research Gate.
Software (free-ware and open-source) for automated respirometry can be found at http://www.AquaResp.com - a new version written in Phyton will be available soon. This version will be able to run on PCs, Macs as well as Raspberrry Pi
John Fleng Steffensen
www.mbl.ku.dk/JFSteffensen
Hi Prof Steffensen,
Thanks for your nice answer.
Thanks again for giving me your valuable time and answered my question.
All the best,
Pedram
Dear Pedram,
In addition to John Steffensens many excellent papers on fish respirometry, you may want to read this recent review:
https://www.researchgate.net/publication/248396411_Aerobic_scope_measurements_of_fishes_in_an_era_of_climate_change_respirometry_relevance_and_recommendations
Here we go through some of the recent advances in aquatic respirometry, such as optodes, and discuss different techniques for maximum metabolic rate measurements. We also discuss the relevance of aerobic scope for different fish life strategies, as well as for environmental challenges.
Best regards
Fredrik
Article Aerobic scope measurements of fishes in an era of climate ch...
Hi Fredrik,
Yes, I have studied your review article recently and found it really useful in this field.
All the best,
Pedram
- Badges
- Science method
- Similar topics
- Biological Science
- Ecology