After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

How many kind of Contrastive loss are there? What is the significance of each kind?

Hello all, It would be kind of you to help me find out what are the different types of contrastive loss functions that currently available and their significance regarding different aspects i.e....

27 September 2020 2,993 2 View

How Hansen solubility parameters helps in drying fibers during electrospinning?

Hansen solubility parameters determine hoe a polymer is soluble in a the ifferent solvent. But how it helps in case of solidifying a fiber during electrospinning process?

14 September 2020 4,570 2 View

Is it possible to use MAC address of a device as an authentication key for accessing a file?

is it possible to use a MAC address of device as an authentication key for accessing a file? can this be implemented manually ? like if I want a specific device to access a file( i have no access...

22 July 2020 2,667 15 View

Can two index number be employed in Gmsh?

Hi I am trying to build an square lattice array of perforation on a disk surface. I like to create a for loop inside a for loop. One for x positions (loop 1) and other for a y position for all the...

17 June 2020 2,610 1 View

What kind of assessment can be conducted to find the changes in terms of protease or proteolytic activity?

I want to observe the changes in terms of protein breakdown during germination. Few techniques are known such as Colorimetric, fluorescence assay to assess protease activity. Which one is the best...

23 January 2020 9,487 1 View

How does even Majorana Fermion exist ?

How does even Majorana Fermion exist ? Why any fermion can be written as a combination of two Majorana fermions? Is there any physical meaning in it? Why Majorana fermion can be used for...

26 January 2019 3,017 3 View

What will be the net force equation for a SISO radial AMB system?

I have a SISO system of active magnetic bearing, i/p of which is current and output is the combine displacement in x and y. the normal equation for force of both axis is like this :...

19 August 2018 6,129 1 View

Decrease of Corss Polarization Level?

What is the procedure to reduce the cross polarization level for a given antenna? Is there any general guideline?

08 May 2017 9,946 1 View

'Biomedicine Spray-On RNA Protects Plants from Viruses for Weeks' a news in nature plants 9-1-17. Will not RNase enzyme be a problem there?

RNase enzymes are everywhere. Therefore, there should have possibilities to degrade RNA that are sprayed to plant.

18 January 2017 3,450 7 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

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I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

Dear Colleagues, I am looking for a way to dop of electrospining nanofibers on the HEPA filter.Someone can guide me?

Electrospining method is important for production of nanofiber .If doped with nanoparticles ,can be used for remove of contaminant such as VOC , but don't have mechanical strengh . For finding a...

24 January 2021 7,290 3 View

How to measure the hardness for PLA & PHAs polymers?

Hello, I am a bit confused on whether to follow ASTM D785 (Standard Test Method for Rockwell Hardness of Plastics and Electrical Insulating Materials) or ASTM D2240 (Standard Test Method for...

21 January 2021 7,487 4 View

Why the bandgap of CH3NH3PbCl3 films are different for spin-coating method and electrospinning method?

The observed bandgaps are 3.8 eV and 3.4 eV for spin-coated and electrospun films, respectively. What are the possible reasons?

08 January 2021 5,646 6 View

How to get phenol(BHT) back to organic phase from acidic aqueous layer?

07 January 2021 6,527 3 View

LC-MS/MS for n- hexane and DCM extract of seeds?

I prepared my seeds extracts in hexane and DCM, now I facing problems in LCMS/MS, it blocks the system, there is any best or ideal mobile phase for both non-polar extracts so that nonpolar...

20 December 2020 2,404 3 View

Blow extrusion of PLA with the HDPE non rotary die. Is it possible?

Hello, I managed to run a small blown extrusion line, head diameter 70mm / screw 45mm. The machine is to be used for extrusion of sleeves from 200 to 500mm wide. Unfortunately, I have a draw...

13 December 2020 2,824 5 View