I want know is there any code/program which give the count of Vehicle (Moving object) in matlab?

I have completed my noise monitoring and videography ( for traffic volume count) survey ,And I just want to count vehicles moving on road , So let me know is there any code or program in MATLAB to...

24 December 2020 3,316 1 View

One way or Two way ANOVA?

Dear All, I have one dependent variable and three independent variables and wanted to apply ANOVA as a measurement. However these variables do not have any levels or categories. Please suggest...

01 December 2020 2,069 8 View

How to Identify the tuples fall in False Negatives in Confusion Matrix?

All metrics to measure predictability are simply depend(Negatively) on False Negatives(FN) as well as False Positives(FP). Is there any way of knowing who(Instances/Tuples) went to FN basket as...

08 November 2020 192 3 View

Here we are fabricating Perovskite solar cell in air atmosphere, even for standard cell also not getting PCE more than 1.5% but in reports PCE is 15%?

Hi, I am PhD student working on Perovskite solar cells (PSCs), I have fabricated standard cell (FTO/C-TiO2/M-TiO2/CH3NH3PbI3/Spiro-OmeTAD/Ag) in air atmosphere. but i am not getting any...

06 October 2020 8,168 8 View

I want to simulate a VAR model. How do I choose the coefficients to ensure stability?

I know how to compute eigenvalues to test stability. The question If i have for example a 10 node model with 5 lags, how do i choose stable parameters without just guessing.

06 October 2020 5,238 6 View

Best techniques available for the VOCs adsorption by MOFs?

I am very new to MOF-field. I really need your help, can anybody please suggest me which is a simple and best technique to do VOCs adsorption studies for MOFs? In our lab we have GC-MS and my big...

05 October 2020 8,874 2 View

The ratio of hypocholesterolemic/hypercholesterolemic (h/H) fatty acids?

The following equation is generally used for the calculations of the ratio of hypocholesterolemic /hypercholesterolemic (h/H) fatty acids, or h/H fatty acids index (Santos-Silva et al. 2002;...

24 September 2020 9,939 5 View

Stagetips for desalting small molecules (not peptides)?

I work with derivatized neurotransmitter molecules. I was using C-18 SPE cartridges previously for desalting them before LC-MS, but now I would like to adopt in-house made stagetips. These are a...

22 September 2020 4,854 6 View

Is there any quantitative measure of Interdependence between different data-variables ?

In my observed data I have different data variables(12 in Number) , Let say X1,X2......X12..I want know ,How one data variable influence the value of another data variable.. is this...

11 May 2020 2,968 1 View

How to design a Spaceborne Antenna for HEO (Highly elliptical orbit) satellites? What types of Antenna are useful for this purpose?

How to design a Spaceborne Antenna for HEO (Highly elliptical orbit) satellites? What types of Antenna are useful for this purpose?

29 April 2020 9,604 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View