Hello Richard,

when I used alphaGA for my experiments, the stock solution was prepared in DMSO at 100 mM and kept at -20°C. Then I diluted 1:1000 in culture medium to obtain a working solution at 100 uM.

The alpha GA does not dissolve well in solutions and you often see the powder in the bottom of the tube; so you need to:

sonicate the defrosted stock solution for 3-5 minutes,

dilute in medium and again sonicate (3-5min) the working solution before to apply on your cells.

Remember also to vortex well before and after sonication to help the dissolution of aphaGA.

I hope my suggestions are helpful.

Bye

Davide

Thanks a lot for these datasheets Ali and for your detailed protocol Davide. 

By the way Davide, it is cool to get an answer from you by using Researchgate. So unexpected ! And of course you gave all the accurate informations I needed.

Best.

Richard