Hi Katherine

Surface sterilization usually doesn't work against endophytic contaminants. In addition, barley, (and maybe also saltgrass) bears bracts attached to the grain coat and that constitutes another protection for resisting spores.

However, you can use clotrimazole, myconazole or any normal antimycotic drug to get rid of the fungal contaminants.

Even if you are using benomyl, I'm pretty sure your contamination is not basidial.

good luck

Francisco

Hi, You can use cycloheximide, its work against all kind of fungi & yeast.

Seed lots can vary in endophyte isolation frequency. Try a different lot in which you might find some endophyte-free seeds.

Use nystatin, add by filtration to media at 55 ºC.

Greetings

https://phytotechlab.com/nystatin.html

Natamycin (=pimaricin) provides broad spectrum antifungal activity.

I think plant cells are sensitive to cycloheximide, as are most eukaryotes.

Commercial fungicidal seed treatments may also help: http://www.apsnet.org/edcenter/advanced/topics/pages/cerealseedtreatment.aspx

Hi Katherine

I agree with Oadi Matny

Dear Kat.

In the market, Phytotechnology offer an additive called PTC3, is added before autoclaving media and controls good the growth of contaminant microorganisms.

Greetings.

Thanks Rodrigo, I'll have a look!

Actidion (cycloheximide), if growth of other eukaryotes is not required

Amphotericin B

Our drug is Incanon, if it is necessary to suppress the growth of fungi, but plant or animal tissues must grow )