I was wondering about cutoff values for BLAST score and p value. Is there any reference for their cut off values?
Dear All, I want to perform KEGG enrichment analysis for some of my genes. Kindly suggest me any useful database or way to perform it. Thanks in anticipation.
02 March 2016 6,796 2 View
To determine the antigenesity of a bacterial protein, we clone, express and produce antibodies against that protein. But what can be possible ways to determine the virulence of a bacterial protein?
11 December 2014 7,957 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...
31 July 2024 6,464 5 View
I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...
28 July 2024 7,827 6 View
The literature on Supply Chain Integration (SCI), Supply Chain Management (SCM), and collaborations, spanning several decades, deserves special attention. This topic, although addressed for a long...
23 July 2024 5,672 3 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
Hello, when calculating the p value for the alleles in the table, how do we place the values in the chi-square test in the four-eyed table? Thank you very much for your attention.
17 July 2024 998 2 View
I'm currently working on the implementation of pulses (legumes) in bakery applications. In one case, pulse flours could be added to bread to increase the nutritional value. Pulses (in particular...
17 July 2024 7,293 3 View
We have conducted a systematic review to investigate the effectiveness of a treatment for a psychological disorder. We aim to report effect sizes and p values of the reviewed studies but one study...
10 July 2024 7,186 4 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
I need some articles on the analysis of the nutritional quality of barley (H.vulgare.L) and the analytical approach of metabolomics and proteomics.
26 June 2024 3,451 2 View