I experienced considerable amount of cytoplasmic vacuolization in my HepAD38 cells after recent passaging. What indicates this? Is there any method to reverse the aberrant morphology of HepAD38 Cells?
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
Hi! I have performed several immunofluorescences of cultured ARPE-19 cells with different antibodies expecting in each one a cytoplasmic localization. However, it seems to localize in the...
06 June 2024 7,764 1 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
It can be found in most literatures and reviews that METTL3 exerts its methylase activity in the nucleus, interacting with adaptors such as METTL14 and WTAP, but is it possible for METTL3 to play...
09 April 2024 9,801 0 View
I stimulated RAW264.7 using LPS(100ng/ml) + IFNgamma(20ng/ml) for 24h,then using WB to detect CD80,CD86 and inos,inos is obviously increased but CD80 and CD86 are decreased.What is the reason for...
22 March 2024 1,636 2 View
What is the bubble in the cytoplasm as the figure I attached below? It also can be released into the culture medium after the cell dead.
18 March 2024 1,410 0 View
I am looking for the paper or the persons who worked on cloning of short sequences targeting the different cellular organelles (vacuole, mitochondria, plasma membrane, septum) in plant pathogenic...
11 March 2024 1,878 0 View
We are right now researching a mitochondrial protein and intending to find the proteins it interacts with. Therefore, we did CoIP and MS/MS. Interestingly, several proteins are cytoplasmic...
31 January 2024 3,192 2 View
How to perform Western blot for GPI-anchored proteins, and are there any differences in the experimental conditions, particularly in the extraction and processing procedures of protein samples,...
28 January 2024 4,280 0 View
In an experimental rat protocol, I am observing frequent vacuoles in the cerebral neurons, almost exclusively in the nuclei. They occur much more frequently in some experimental groups compared...
28 November 2023 4,866 3 View