What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

What are the parameters that plays a role in life-time degradation models of concrete? what are the most important tests to measure these parameters?

I'm working on a research about the prediction life-time models for concrete in harsh environment. I have to answer those two questions.

14 November 2020 2,043 7 View

Does anyone know of historic sites that have artist in residency programs?

A research assistant and I are conducting primary research on artist in residency programs (artists, writers, etc.) and the use of contemporary art at historic sites to reinvigorate or...

27 September 2020 9,355 4 View

What is PROSPERO - University of York regarding Systematic Literature Reviews?

I have seen for the first time a systematic literature review and meta-analysis that has prospectively registered their review in PROSPERO and I am wondering what is the main reason for creating...

04 August 2020 4,167 4 View

What causes the copy detection limitation in PCR?

Hi all, I wish to understand why doesn't all polymerase can detect a single copy of region of interest? While some polymerase is more sensitive (like dreamtaq), and some are less sensitive, what...

02 June 2020 4,397 21 View

Evidence-based resources for helping kids cope with debilitating & likely fatal disease?

A colleague asked me for any evidence-based approaches to helping kids (and parents) cope with a life-threatening disease, Duchene muscular dystrophy. The child doesn't know he is sick yet. Does...

24 March 2020 2,046 3 View

Does anyone has experience with NEB "time-saver enzyme" overnight digestion?

Hi all, I used the Thermo-ANZA series for overnight digestion of plasmid backbone to ensure clean digestion without any problem. How about NEB? The website said all NEB "time-saver enzyme"...

24 March 2020 7,636 2 View

Difference between cell culture plates and flasks?

In the lab we always use culture plates (33, 60, 100, 150mm) for passing cells and conducting all sorts of experiments. I wonder what is the perks of using cell culture plates v.s. flasks? Should...

12 March 2020 1,361 2 View

How can can I find a none fee charging credible journal to publish a co authored paper?

I am looking for a none fee charging journal to publish a coathored paper. Its in special education

20 January 2020 5,203 3 View

Can I transfer Spheroid to normal attachment plates for other assays? How will it affect the morphology and consistency?

Do you guys have any experience in transferring spheroid produced in low attachment plate to a high attachment plate after spheroid production? Would it affect the morphology of the spheroid? I...

11 December 2019 1,918 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

No title

01 March 2021 3,905 5 View