What happens to the shape of staph aureus when they are treated with antibiotics?

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XPS peak fitting: Must satellite peak areas be accounted for, while calculating oxide thickness?

Subject: XPS peak fitting with satellite peaks I am currently working on peak fitting Sn3d spectra that include satellite peaks (see attached figure). My aim is to calculate oxide thickness by...

02 October 2023 3,791 1 View

Diesel Heater Glow Plug actuation using MOSFET Trigger Module?

Can I supply 12V DC voltage to diesel engine glow plug through a MOSFET Switch module.

03 June 2023 7,983 1 View

Can i actuate a GDI injector using arduino and motor shield combo ?

I had a plan to run the GDI injector for a single pulse injection. This injector draws high current and voltage for its operation. For that I used a GDI injector, Arduino Atmega 2560, Motor...

17 February 2023 256 4 View

Presence of grossular-andradite (grandite) series garnets in nepheline syenite gneiss indicate any carbonate related hydrothermal phenomena?

Dear all, I have nepheline syenite gneisses, consisting of grossular-andradite (grandite) series garnets. Other minerals of these rocks are nepheline (seldom altered), plagioclase, k-feldspar,...

21 January 2023 2,819 11 View

Which type of injection system is used in ISRO's VIKASH Rocket engine ?

What is the mechanism of fuel and oxidizer mixing used in ISRO's designed VIKASH Rocket engine?

04 July 2022 9,750 2 View

How can I calculate the excited state energies S1 and T1 in order to calculate delta(EST) energy gap for TADF calculation?

I am using Gaussian 09. I need to calculate delta(EST) energy gap for TADF calculation (for organic molecules).

07 May 2022 5,973 1 View

Channel Estimation for MIMO channels using RIS?

It is known that for optimal RIS control, perfect CSI of all the links between BS and MS through the RIS is required. Therefore, channel estimation and corresponding message feedback methods will...

02 February 2022 7,604 6 View

Is it possible to detect the somatic mutation in serum/plasma of brain tumor after resection and before start of adjuvant therapy using NGS?

We are interested to detect somatic mutation in serum/plasma of brain tumor patients, in samples collected after resection and before start of adjuvant therapy. Is it feasible to get the somatic...

21 December 2021 1,968 5 View

Business model and organizational structure- how are they related?

How does business model of a firm influence its organizational structure? Any pointers are highly appreciated. Thanks a lot. Does the business model get defined based on the structure? I doubt...

08 November 2021 5,037 22 View

Easiest way to measure particle size distribution of Graphene/GO dispersion?

What is the easiest way to measure the PSD for GO/or Graphene/ CNT dispersion? Something quick and easy and cheap NOT by using SEM or other imaging analysis

26 October 2021 1,974 8 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View