Agarose gel electrophoresis
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
Dear RG Community, I am looking for some suggestions of good references wherein the cyclic deformation behavior (i.e. cyclic stress-strain curves and evolution of stress-amplitude with the number...
29 May 2024 3,202 4 View
How can we simulate TSV in the Silvaco example? Exactly, I need the instructions for the attachments file. Best regards.
29 May 2024 1,638 1 View
Hello, I have a methanol extract that I have let stand for 3 days, three times. I have evaporated the methanol and, just to be sure, mixed it with water and lyophilized it. Before performing HPLC,...
20 May 2024 1,847 13 View
Hello, After synthesis curcumin metal complexes, how can I purificate molecules with 1:1 and 1:2 metal-curcumin stoichiometry, and is there any reference study on this matter? In the literature,...
04 April 2024 8,571 4 View
Hello, In the literature, there are some MS/MS results that include hypothetical proteins, which can be shorter than 40 amino acids. I can also find these when I search for an organism in the...
04 April 2024 5,098 2 View
Dear Experts I downloaded and installed the Desmond Schrodinger software in my Linux system. installation was fine and when i tried to prepare the protein in protein preparation wizard I noticed...
28 December 2023 1,695 1 View
Hello dear, I request you all please guide me how to resolve this issue. I dual booted my pc and created the ubuntu and windows as separate OS. Thereafter followed the guidelines and installed...
16 December 2023 6,189 4 View
Hello, I've recently started exploring molecular docking applications, and I'm still in the early stages.I'd like to ask which proteins should be considered when examining the antimicrobial...
03 December 2023 737 4 View
Hello, I've recently started exploring molecular docking applications, and I'm still in the early stages. I'd like to ask Can I choose a ligand by giving the amino acid sequence and then do...
03 December 2023 3,074 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,478 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,952 0 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,129 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,032 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,672 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,404 6 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,743 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 486 0 View
