Could you answer some basic questions on CRISPR-cas9?

Hi, I would like to ask simple some questions related to CRISPR-Cas9 that I could not understand by reading some basic articles on it. I am not in that scientific field so my questions might be...

21 October 2020 2,439 2 View

Non-specific optical stimulation of parvalbumin (PV) neurons - Any possible causes?

Hi all. Recently, I encountered a problem regarding the optogenetic stimulation experiments. In our study, we are using the blue laser (473 nm) to optogenetically stimulate parvalbumin (PV)...

05 February 2020 8,253 11 View

How can I calculate band gap of nano metal particle?

I'm studying for nano metal particles. The metal particles diameter is about 5nm. And the particles are stuck on nano wires through UV irradiation. I mean the nano wires are soaked in metal...

08 January 2020 8,807 6 View

Bicrystal fabrication company?

Hello. I am looking for a bicrystal fabrication company. My major is focusing on the exploration of grain boundary structure. And thus for a model experiment I need metal*or ceramic**...

08 January 2020 4,038 3 View

Does anyone have experience in using Doric Neuroscience Studio for fiber photometry experiments?

Hi. I am a graduate student using Doric Neuroscience Studio. Since there seems to be not much references about using Doric's fiber photometry console(FPC) (v. 4.x or 5.0.0) and our lab doesn't...

27 June 2019 9,512 2 View

Is codon optimization necessary for human TDP 43 expression in Drosophila melanogaster?

Dear specialists in TDP 43 researches in Drosophila Melanogaster I actually constructed human TDP 43 in pacu2 vector(uas promoter included) and injected it into Drosophila. and checked how it...

13 October 2018 2,149 1 View

DOTAP + CpG ODN protocol?

Greetings, I'm trying to stimulate isolated primary B cells using CpG ODN and DOTAP mixture. However, because I've never used DOTAP, it seems I'm having a difficulty to make a proper DOTAP +...

19 March 2018 9,136 2 View

Does anyone have experience with mouse tail cross section( cryosection or paraffin section)?

If yes, could you please advice a protocol? Thank you in advance for your kind help.

29 June 2016 9,350 2 View

Is it possible to make p-doped reduced graphene oxide?

22 September 2015 7,856 3 View

Can you answer my questions on native-page composition?

29 October 2014 4,283 3 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

What is the system of units used in the thermal conductivity by Spitzer and Harm?

23 February 2021 9,253 1 View