Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Measuring viscosity and density under high pressure?

I need the density and viscosity of a molecular fluid under relatively high pressures, I expect something around 500 MPa. This kind of measurement is way outside my field, and I can find only very...

19 October 2020 2,812 3 View

"Internal solution magnitude limit was exceeded". How to solve this question in Ansys Workbench?

I am modelling a foundation connection to a wind barrier. The connection is 4 prestressed bolts with an anchorage plate in the bottom, it is all cealed in a clump of concrete. When I change the...

29 October 2019 4,944 2 View

Does anyone know an easy and simple method of detecting trichloroacetic acid (TCA) and/or trifluoroacetic acid (TFA) in solution?

I am investigating electrokinetic migration of these acids across a supported liquid membrane (SLM), and need to determine their presence (if any) in the aqueous acceptor phase. I am thinking...

17 October 2018 9,594 4 View

How to add response bias into a repeated measures mixed anova model?

Hi everyone! I have a 2 repeated measures that was asked at 3 moments in time (within-subject factor). We doubled the measure to control for response bias. So the measure (likert scale) probed...

02 April 2018 2,389 3 View

Oscillating behaviour in ITC data - any explanations?

Hi, When doing ITC in our lab we have recently observed an osculating behaviour of the raw ITC data. We have tested different systems and the behaviour is the same (however, most visible with...

06 March 2018 1,117 6 View

SaOs-2 cells and lipofectamine-2000?

20 April 2016 1,781 3 View

BiFC and ImageJ threshold?

Hi, I'm currently working on the dimerization of two solute carriers. For that I'm using bi molecular fluorescence complementation. I've transfected 293FT cells attached to poly-D-lysine coated...

21 February 2016 4,440 3 View

How can I calculate functional hill numbers?

I would like to calculate the functional diversity measures proposed by Chiu & Chao (2014). The paper gives formulas for the calculation of functional hill numbers, mean functional diversity...

08 June 2015 9,731 7 View

Are there any well-documented learning algorithms for MLPs with only positive weights?

I have a 3 layer MLP architecture with which I want to approximate models for certain logics. I know that these models can be approximated using only positive weights and this property of the...

24 February 2015 4,079 15 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View