After slaughter oxygen supply to muscle is stopped so glycogen is metabolized following anaerobic pathways, aiming to generate the needed ATP for keeping the equilibrium in the muscle cell. As consequence energy sources (creatine phosphate, glycogen, glucose, ATP, ADP and AMP) are depleted and lactic acid (from glycogen) and hypoxanthine (from ATP) are produced. Lactate is accumulated leading to a drop in the pH of the muscle. The ability of the muscle to generate post-mortem energy is known as glycolytic potential and can be determined by measuring the content of glycogen, glucose, glucose-6-P and lactate. A direct relation exists between the rate of lactic acid production, the final amount of lactic acid, final pH of meat and the meat quality. Fast lactate production and final low pH are commonly related with PSE (pale, soft and exudative) meat; when low rates of lactate production and high final pH are observed DFD (dark, firm and dry) meat is obtained; both are considered as low quality meat. A correct glycolytic potential along with adequate glycolysis ratio determine the final quality of the meat. Initial amount of glycogen and the rate of conversion into lactate will determine the quality of the meal.
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The main factors which affect t the sensory quality of meat as general include; the type of meat, warmed-over flavour, production and rearing conditions and postmortem practices. However, there are many other factors which have effects including Genotype which affects juiciness, tenderness, acidulous taste , sex which affects juiciness and acidulous taste, a prolongation in ageing time which affects the colour and taste intensity.
The factors affecting the quality of meat are different, the most important of which are microbiological factors, microbes, fungi, physical factors such as cooling, freezing and chemical factors.
Quality of meat is affected by the genetic propensity of the animal, how the animal is reared, and the nutritional status during production. These factors affect the fat, lean and connective tissue component of meat and therefore influence meat quality.
Short-term acute stress, such as excitement or fighting immediately prior to slaughter, produced lactic acid from the breakdown of glycogen. This results in meat which has a lower pH, lighter color, reduced water binding capacity, and is possibly tougher.