I am trying to test the effect of a new drug on cancer cell line combined with a subleathal dose of Cisplatin. Is the subleathal dose, the dose that doesn't have any effect on the cells? or the dose that is just less than the IC50?
I think that sublethal dose is the dose that preserve cell viability so no effect on cell survival after treatment. A sort fo analogue of NOAEC based on viability. IC50 is the toxic concentration that kills 50% of the cells so it can not be considered sublethal because 50% could be considered moderato to hig toxicity.
I know the IC50 is not a subleathal dose. Actually I was asking if the dose that kills let's say 10% of the cells subleathal or not?
but I think the first part of your answer cleared that. The subleathal dose must have no effect on the cells viability at all.
Thank you
the dose that is just less than the IC50 is sublethal dose. In this case lethality is required feature...
i didn't understand your answer. If my IC50 is 50 mg/ml and the dose that doesn't cause any apoptosis is 3 mg/ml. what is the subleathal dose?
The wide difference in answers is surprising!
One definition of Lethal Dose would come from a usually reputable source:
https://www.drugs.com/dict/lethal-dose.html
"Definition: the dose of a chemical or biological preparation (a bacterial exotoxin or a suspension of bacteria) that is likely to cause death; it varies in relation to the type of animal and the route of administration; when followed by a subscript (generally “LD50” or median lethal dose), it denotes the dose likely to cause death in a certain percentage (50%) of the test animals; median lethal dose is LD50, absolute lethal dose is LD100, and minimal lethal dose is LD05."
A sublethal dose would therefore have to be well BELOW the LD05. In clinical use a sublethal dose would be a dose of a toxic substance that is "insufficient to cause death." The therapeutic dose for most substances would then be well below even the sublethal dose. Having a dose that causes death in 1 out of 20 patients would be a horrendous overdose.
I could not find a reputable source that shows how you would measure a sublethal dose, but as I am a clinician I would easily miss sources that address cellular work. Sorry!
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