Diego Baderna

10 Questions 52 Answers 0 Followers

Questions related from Diego Baderna

Benchmark dose: where I can find data/repository for this property?

Dear colleagues, our health institute in Italy recently released a guidance for risk assessment of chemicals in which the benchmark dose must be used to assess the risk (as margin of exposure) of...

26 January 2021 2,000 3 View

Are there some experts on dyes structures?

We are evaluating aquatic toxicity of some dyes (most of them are for textile and leather) using bioassays but we would like to have an experct check of the structures of the compounds we are...

04 October 2016 2,034 14 View

Reference doses or Admittable Daily Intake for drugs for human toxicity studies?

Dear All, We are looking for reference doses and/or admittable daily intake for human and veterinary drugs in order to evaluate the effects of drugs residues and metabolites found in...

31 May 2016 7,417 5 View

Micronuclei and Comet assasys in A549, HepG2 and BEAS-2B cells. Can someone help me sending a valid protocol and some advices?

I would like to perform the micronucleus and comet assays in our cells after exposure to the mixtures we are investigating. In literature there a lot of protocols with big differences in reagent...

07 January 2016 3,489 3 View

How is the variability associated with the MTT/MTS assays?

Dear all, one of my colleagues asked me a curios question. She would like to know what is the experimental variability of MTT/MTS assay on human cells because she is creating an in silico models...

08 September 2015 8,387 2 View

How do I derive RfD from RfC for ingestion and inhalation?

Dear All, I'd like to calculate Reference Dose (RfD) from Reference Concentrations (RfC) to be used in the risk assessment for ingested and inhaled compound. For inhalation we work as follow RfD...

09 July 2015 796 6 View

Is anyone familiar with transfecting A549, HepG2 and BEAS-2B cells with a GADD45a luciferase reporter?

Dear all, we would like to transfect our clones of A549, HepG2 and BEAS-2B cells with a GADD45a luciferase reporter plasmid that will be sent from a research team in China. This research team...

09 April 2015 832 2 View

How can I sterilize tubes in glass jars?

back from the summer holidays a doubt has arisen.Do I have to sterilize 1.5 Ml PLASTIC tubes (the eppendorff) and I have some glass jars with lid (jam jars).I have to put them in the autoclave...

02 September 2014 5,634 3 View

What does a PBS of 0.1 M mean?

I have decidet to bought Rhodamine 123 to evaluate mitochondrial activity of a treated green algae. In the research (published) paper where the protocol is described, it is written that I have to...

02 September 2014 1,302 4 View

What are some software/tools for LC50 determination using the probit or trimmed Spearman-Karber method?

I' d like to calculate lethal concentration (LC50) or effect concentration (EC50) in our study with daphnids. The US EPA suggest to derive values using both the probit method and the trimmed...

04 June 2014 6,260 20 View