Diego Baderna

5 Questions 32 Answers 0 Followers

Questions related from Diego Baderna

How is the variability associated with the MTT/MTS assays?

Dear all, one of my colleagues asked me a curios question. She would like to know what is the experimental variability of MTT/MTS assay on human cells because she is creating an in silico models...

08 September 2015 8,339 2 View

How do I derive RfD from RfC for ingestion and inhalation?

Dear All, I'd like to calculate Reference Dose (RfD) from Reference Concentrations (RfC) to be used in the risk assessment for ingested and inhaled compound. For inhalation we work as follow RfD...

09 July 2015 740 6 View

How can I sterilize tubes in glass jars?

back from the summer holidays a doubt has arisen.Do I have to sterilize 1.5 Ml PLASTIC tubes (the eppendorff) and I have some glass jars with lid (jam jars).I have to put them in the autoclave...

02 September 2014 5,574 3 View

What does a PBS of 0.1 M mean?

I have decidet to bought Rhodamine 123 to evaluate mitochondrial activity of a treated green algae. In the research (published) paper where the protocol is described, it is written that I have to...

02 September 2014 1,241 4 View

What are some software/tools for LC50 determination using the probit or trimmed Spearman-Karber method?

I' d like to calculate lethal concentration (LC50) or effect concentration (EC50) in our study with daphnids. The US EPA suggest to derive values using both the probit method and the trimmed...

04 June 2014 6,202 20 View