Osama Elkashty

10 Questions 15 Answers 0 Followers

Questions related from Osama Elkashty

What statistical test would fit here?

I have 3 groups with 3 time points where I measured wound healing. What statistical test would measure the significance between the data? I thought oneway ANOVA between groups in each time point...

03 August 2022 4,532 0 View

Masson Trichrome colors?

What is stained blue and what is stained red exactly in bone with masson trichrome? I read different descriptions. Can I have a reference too?

17 January 2022 9,564 0 View

How to measure immunohistochemial intensity not area size using imageJ?

I have a set of photos that are calibrated to microns and I need to measure the immunohistochemial intensity ( brown ) difference between them using imageJ. Any suggestion on how? Should I use...

18 November 2021 3,491 0 View

What is the dose of Cisplatin that should be given to mice to be comparable to human dosage?

I am using NU/NU Nude (Crl:NU-Foxn1nu) mice as a xenograft tumor model and I will need to inject the Cisplatin by intraperitoneal injections either alone or in combination with a newly tested drug.

22 August 2018 1,907 2 View

What exactly is the sublethal dose of a drug? and how i measure it?

I am trying to test the effect of a new drug on cancer cell line combined with a subleathal dose of Cisplatin. Is the subleathal dose, the dose that doesn't have any effect on the cells? or the...

25 February 2017 9,501 6 View

I tried to make tumorsphere assay in low attachment 6 well plate but i got most of my cells attached after 3 days. any solution for that ?

i use head and neck cancer cell line. using media DMEM-F12 with 2%FBS, 2%N2 and 1%B27. my seeding density is 20000/well in 1.5 ml media.

24 November 2016 7,274 4 View

How to add a drug for 3 days only on the cells in tumorsphere assay?

i will test some drugs on the ability of forming tumorsphere with my cancer cell line. I know i will add the drug the next day of seeding to the media, but how to remove it after 3 days without...

21 November 2016 8,788 2 View

How should i treat the unsorted cells to be compared to the sorted cells passing through the FACS?

I will sort some cells using FACS sorting and I need to compare them to unsorted cells. Do I have to make the unsorted cells pass through the same steps like the sorted one but without staining to...

03 October 2016 2,792 5 View

I need to do the Transwell migration and invasion assay on squamous cell carcinoma cell line. anyone know the protocol and where can i get the plates?

working on SCC12 cell line where i can get the Matrigel?

27 August 2016 3,960 2 View

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)?

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)? need to get 5 fluorouracil as a chemotherapy for SCC

10 July 2016 2,625 5 View