How can I know which family/species of virus is making the difference between my samples?

I have several metagenomic samples, from which I got the viromes through a bioinformatics pipeline. I made a PCoA of those samples, and some of the samples cluster together. I would like to know...

08 July 2020 8,434 2 View

If female mice are put together on the same cage, are all of them on the same estrous cycle stage?

I work with female mice and I have this doubt. If they are all put together on the same cage, are all of them on the same estrous cycle stage? If so, is there any literature that supports...

01 April 2020 6,266 10 View

How to blast a paired end fastq file?

Hello everyone, I have a paired end fastq file and I know that BLAST+ in command line, accepts fasta format. But I don't know how does it work for a paired end fastq file (I mean in two different...

22 May 2019 2,482 2 View

260/230 ratio of my RNA samples are very low. Is there any method to try purify the sample and synthesize cDNA!?

I extracted RNA from spinal cord tissue using Trizol and following invitrogen's protocol for RNA extraction for PCR. I had good 260/280 ratios (around 1,8-1,9), but very low 260/230 ratios...

16 April 2019 768 2 View

How to perform KASP genotyping of zebrafish larvae at 72hpf?

Dear all, I need to genotype some zebrafish larvae at 3-4dpf. I currently tried to perform it as I do it for the adults, but it did not work. Basically: 1. Fin clip and add the fin to a 96 well...

20 January 2019 989 3 View

IHC in peripheral blood smear?

Hi everyone. We want to do an IHC in peripheral blood smear but it's our first time, and we have some doubts. How do we have to fix the samples? Which is the best fixation agent, acetone, methanol...

15 October 2018 9,338 5 View

Non-transcriptomic pathways that can increase lipid accumulation?

I am currently doing a project investigating the lipid accumulation potential of a chemical using an in vitro model. Upon observing significant lipid droplet accumulation at various...

18 March 2018 4,812 4 View

Are you studying affects toward science, technology and/or mathematics in university and preuniversity students in your country?

We may share instrument and compare data with ours.

02 January 2018 1,064 12 View

QPCR stopped working for well-set up amplicons

Hi all, I ask for your help to solve a difficult problem I'm experiencing with some samples. I'm performing ChIP-qPCR of a transcription factor. We have done this experiment many times and it has...

29 June 2017 6,286 3 View

Is there a protocol to show cytoskeleton disruption in cells after being treated with cancer drugs?

22 January 2017 8,949 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View