You can use distilled water as a solvent to dilute honey. It is safe to use, but it will speed up the fermentation of honey, so it must be consumed directly, or you can use isopropanol solvent.
You can use distilled water as a solvent to dilute honey. It is safe to use, but it will speed up the fermentation of honey, so it must be consumed directly, or you can use isopropanol solvent.
In the disc diffusion method, it is important to use a solvent that does not interfere with the antimicrobial activity of the honey being tested. One edible solvent that can be used to dilute honey for the disc diffusion method is sterile distilled water. It is a commonly used solvent in microbiology and is often used to dilute solutions and samples for testing. It is safe for consumption and does not interfere with the antimicrobial properties of honey. Besides, there are also other edible solvents including ethanol and propylene glycol, but it's important to note that these solvents may affect the antimicrobial activity of the honey being tested.