What are the best references for an approach to teaching Differential Equations?

Differential equations are known to be potential tools for modeling and predicting recurrent phenomena in time and space, whether simple or more elaborate models. In this sense, how to approach...

18 December 2020 1,750 3 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

Recombinant Interleukin 6 western blot, non specific bands?

Hi I've been trying to produce some recombinant human Interleukin 6. I sub-cloned the gene for human IL6 into a Promega T7 Flexi vector (native gene no tag yet) and confirmed sequencing and...

09 December 2020 2,340 4 View

How many journal articles should come out of a doctoral thesis?

Is it normal if I publish my doctoral thesis into only one journal article (since my doctoral thesis will be data-heavy and theory-light)? Or are doctoral theses expected to be equivalent to 3-4...

01 December 2020 3,803 14 View

Can the potential of LIPIDMICS improve the diagnosis and understanding of metabolic changes in various neurodegenerative disease (PD, HD, AD, MS, etc?

08 November 2020 7,209 1 View

Can I compare data from ion exchange membranes with data from ion exchange resin when determining nutrient supply rates in soils?

Hi everyone, I am trying to decide if I can compare nutrient supply rates between two different methods of collection. Method A uses ion exchange membranes and method B uses ion exchange resin in...

23 September 2020 4,535 1 View

Why would a validation error (confusion) matrix return null in Google Earth Engine?

I am working on validating a collection of images from a time series in Google Earth Engine and the confusion matrix that results from validation of the trained image v.s. the landcover classes...

09 September 2020 7,246 3 View

How can I increase the length of DNA fragments recovered from QIAamp DNA Micro Kit?

Hi everyone. I'm performing DNA extractions for pools of ~10 individual Daphnia magna (water fleas) using the Qiagen QIAamp DNA Micro Kit. I use a pestle to homogenise the Daphnia before entering...

07 September 2020 9,956 1 View

Why is my Simscape model of a Furuta pendulum not fully controllable?

Dear community, I am trying to build a model of a Furuta pendulum in Simulink/Simscape. Unfortunately, when I try to linearize my model with the integrated Model Linearizer, I get an unexpected...

20 August 2020 4,853 6 View

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Nutritional science has provided functional foods with the required amounts of natural antioxidants, vitamins, fats, proteins, polyphenols, carbohydrates, and other components required to maintain...

03 June 2020 7,377 1 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

How to convert isotrophic Deriche filter into anistrophic?

12 February 2021 5,889 6 View

Hi all, would you suggest good books for Image processing, Computer vision, AI, Deep learning, machine learning, and remote sensing?

I would like to get good books for Image processing, Computer vision, AI, Deep learning, machine learning, and remote sensing. Kindly suggest good authors books for the above topics and its links...

09 February 2021 3,561 11 View

Sound transmission loss (Acoustic Metamaterial)?

My design contains three parts. A silicon rubber membrane is placed between upper and lower part. I want to calculate sound transmission loss by using comsol Multiphysic. There are lots of...

05 February 2021 466 4 View

Does anyone know about vision-guided control of civil structures?

I would like to know more about the following issues: 1. Using images as feedback for the closed-loop control of civil structures 2. Simulating the vision-guided control on the computer without...

03 January 2021 7,566 1 View

Why both Training and Validation accuracies stop improving after some epochs?

I am using data augmentation and decaying learning rate after each epoch. If I don't use data augmentation but keeping callback, then my training accuracy reaches to 99.65% but not validation....

15 December 2020 716 10 View

What are the tools and techniques for making Custom dataset for YOLO?

Dear Research Community. I am new to the field of deep learning based object detection techniques. At this time, I am working on a project which will not use front-facing camera to detect people....

13 December 2020 957 3 View

What is the best machine-learning model for counting overlapping objects?

I am developing a machine-learning model to make accurate and fast counting of metal pipe with difference cross-sectional shapes. Well-define rectangular, triangular and circular shapes are quite...

10 December 2020 2,401 4 View

GAN as Data Augmentation?

Hi, I want to apply GAN as a data-augmentation technique in my model. I am bit confused regarding implementation that how could I give the generated images of GAN to my model on run-time? because...

07 December 2020 4,703 3 View

What is the best Machine learning method to extract collagen bundles features ?

what is the best machine learning method , to segment and extract the length , angle and the size of collagen bundles pictures obtained from the human cornea?

07 December 2020 336 6 View