What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

Hello everybody! so I have a plasmid which has a synthetic intron (IVS) & an internal ribosome entry site (IRES), and I'm thinking to remove them as I'm cloning due to some matters related to...

26 January 2021 7,645 2 View

How we can calculated LOAEL and NOAEL for toxic pathological dose ?

how we can calculated LOAEL and NOAEL for toxic pathological dose

02 January 2021 1,175 2 View

What are the new methods of staining??

what are the new methods of staining??

25 December 2020 4,030 3 View

Hi, I want to run simulation with YASARA for different part of my protein separately but I dont know how to run?

I am working on enzyme engineering and want to run the simulation for my enzyme the open and closed conformation to see the effect of the mutation on my enzyme as its located on the lid. However,...

10 May 2020 2,625 1 View

I am working on COVID19 dataset??

I am working on COVID19 dataset, but, When I tried to convert the data.xlsx file to CSV file, I got an error. Also, i got an error when I upload the CSV file to WEKA. I tried to solve the problem...

05 May 2020 4,879 2 View

How can i define the USRP N210 on pc under windows 10?

How can i define the USRP N210 on pc under windows 10 and i used gnu radio 3.8.. i set the configuration and i make the ping and recived reply successfuly but i cannot define the device and an...

21 January 2020 1,442 2 View

What is the number of measurement required in Circulant and Toeplitz sensing matrix in general in term of N an M in compressive sensing?

what is the number of measurement M (the number of measurement vector that result from multiplication of sparse vector N with sensing matrix (NxM)) required when using Circulant and Toeplitz...

16 January 2020 9,666 2 View

Does Tobramycin have an amorphous structure?

I've been analyzing the crystallinity of Tobramycin by the XRD, it is supposed to have a crystalline structure, but when analyzed, it showed an amorphous structure

25 November 2019 5,145 1 View

I've been trying to test the antimicrobial activity of zinc oxide nanoparticles against P.aeruginosa. They exhibited no activity. Any one knows why?

the nanoparticles were purchased from sigma and they have a size range (35-50 nm). i tried to dissolve it in DMSO ( 5% of the nanoparticle solution) and i also tried to sonicate it for 30...

24 August 2019 9,678 10 View

Combustion Engineering second edition by Kenneth W. Ragland, Kenneth M. Bryden?

I looked for this book literally everywhere but I can't find it, dose anyone have a PDF copy of this book because I really need it...

01 April 2019 6,305 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View