When one is designing primers for a PCR, what E-values should expect when primers do not anneal to random sequences in the genome? My primer set has an E-value of 0.013 for the gene I wish, and 198 for three others. Should I redesign them?
The e-value (or Expect value) is a parameter that describes the number of hits one can "expect" to see just by chance when searching a database of a particular size.
Thanks for the replies. So if my primers anneal to the desired sequence with a very low E-value and could possibly bind to other ones with relatively higher (>100times) E-values, does tha mean that my primers are good? Is this what you were trying to say?
The e-value is used as a convenient way to create a significance threshold for reporting results. When the e-value is increased from the default value of 10, a larger list with more low-scoring hits can be reported. On the other hand, a lower e-value will result in a shorter list with more quality hits.
Yes. As per my knowledge, your primers are good for the desired sequence.
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