How to compare different models with different inputs and data length?

I want to run 5 different models to estimate stream flow. In order to optimize the characteristics of these models I use Taguchi method. So I have to run different models according to the Taguchi...

11 February 2021 7,986 6 View

How to find strain energy release rate GI, GII, GIII in Abaqus?

I modeled a simple crack in abaqus. I get J-integral as output as well as components of stress intensity factor KI, KII, KIII. However, I do not know how to get GI, GII and GIII. I searched in...

04 January 2021 1,783 2 View

What is the best way of moscaicing overlapping images?

I have several images with overlaping areas. I try to mosaic them using rasterio.merge library. But rasterio.merge funtion replaces those overlapping pixels with "nan" values. I then tried both...

29 December 2020 9,849 1 View

How can I check the correlation between a group of features and one feature?

Hello all, I have a dataset that contains some pressure meters in a water distribution network and all valves of the network. Since I do not have the graph of the water distribution network, I...

30 October 2020 2,333 4 View

Any idea why my Western Blot Stripping buffer doesn't work?

I activated my membrane twice in total and hybridized it with two different antibodies. The first antibody was the specific antibody I was looking for and the second one was GAPDH. I then...

29 October 2020 5,530 6 View

Changing TEM mode to TE and TM modes?

Hi there, I would be grateful if someone could let me his opinion about this question: There is a coplanar stripline waveguide excited with a TEM mode. My question is that how to create TE and...

11 October 2020 5,816 3 View

I have problem with my SDS gels in western blot and wondering anyone could help?

So when I prepare my 10% SDS gel for Western blot, I see that my resolving solution is perfect but my stacking solution does not seem to be working properly. So when I take the comb out, the wells...

17 September 2020 3,355 7 View

How can we optimize a water distribution system if we do not have the zones' topology (pipes graph )?

Hello everyone, My issue is about a water distribution system that I am working on a zone of the system where does not exist any plan for the place of pipes. However, we have the place of...

14 September 2020 5,978 6 View

Whats this plant?

Dear All Hi I wanted to know the scientific name of this plant species. would you please help me to identify it. Actually, I found it in the area in the East of Iran.

21 August 2020 6,705 2 View

Which version of Mplus is suitable to assess the exploratory factor analysis of a questionnaire with 20 items?

Dear Researchers and statistical analysts, Which version of Mplus is suitable to assess the exploratory factor analysis (EFA) of a questionnaire with 20 items? I'm working on initial validation of...

14 August 2020 3,341 4 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can any one provide information about medicine, epidemic and critical issues or it is mandatory to be an expert ?

Due to the pandemic of coronavirus, the most source that the people depend on was social media. There is no guaranteed solution that is biased from political opinion. An example of that the...

01 February 2021 7,567 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Is there a way to cut the restriction enzyme only the desired part?

Hello, I am currently making a luc gene. The primer was ordered and the band amplified with pcr was gel extracted. Restriction enzyme was treated to make the insert sticky with the vector......

18 January 2021 4,357 3 View

Questions on data cleaning and imputation in longitudinal studies?

The study involved mathematics students completing a survey with several scales at three time points. 1) What is a reasonable threshold for missing data to remove an observation? Does this...

04 January 2021 5,582 1 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

How to handle missing values?

Dear colleagues, What can you suggest in order to fill the missing values, as you can see in the picture? Best Ibrahim

24 December 2020 4,834 5 View