Distinguished researchers, hello. I have a question for you. Recently, I have been doing research on bacterial exocrine protein, but the protein concentration obtained after washing the supernatant of bacterial solution is low, so the detection is not obvious. Freeze-drying has been considered, but the salt in the medium is also highly concentrated. What are the methods to get a high concentration of protein? thank you
Dear Meiyang Zhao sorry to see that your very interesting question has not yet received any expert answers. As an inorganic chemist I'm certainly not a proven expert in this discipline. However, it might be worth reading the following potentially useful articles. Perhaps they can provide you with some useful hints (although I'm not sure 😳):
A Simple Outline of Methods for Protein Isolation and Purification
Article A Simple Outline of Methods for Protein Isolation and Purification
Fortunately this paper has been posted by the authors as public full text on RG, so you can easily download and print a pdf file.
Also please see:
An optimized system for expression and purification of secreted bacterial proteins
Article An optimized system for expression and purification of secre...
Unfortunately this paper is not freely available as public full text on RG. Just check the Abstract and see if it's of interest to you. Two of the authors of this study have RG profiles. Thus there is a good chance that you can request the full text directly from one of them via RG if the paper is of interest for you..
Good luck with your research and best wishes, Frank Edelmann
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