Can any one explain:
when we contract aorta with KCl 90 mM then washing it after that we precontracted aorta with Nor adrenaline or PE then relaxed with SNP
In some paper used in 100% KCl contraction and some paper used 100% PE or NE contraction
Which one correct
Can't tell exactly what you're doing from the run-on statement you present and the grammar of your question. Both KCl and PE are used to induce contraction (by different routes, obviously) in aorta, and SNP acts as a blocker for the KCl route. It's not a question of one treatment being correct and the other not, they're used as test stimuli to test activity of (especially) the aortal endothelium in these routes, and to test the activity of various drugs at agonizing or antagonizing such contractions.
I could not understand what you exactly need to know, since KCl or Phe are able to contract the aorta. The maximum contraction by the challenge dose is used for the studies.
Regards,
Wilson
To follow up my earlier posting: Mudhir, KCl contraction is commonly used to establish a standard value for contractility for samples, because the absolute response to it is quite constant across many different kinds of contractile tissues (i.e. aorta vs. saphenous vein, with/without endothelium, etc.). See, for example, http://www.pps.org.pk/PJP/2-2/Dileeprohra.pdf
Besides that experimental advantage, there is another reason: the contractile response (to KCl) is a cheap, non-destructive, reversible-effect *normalizing* calibrator, against which the individual responses of various samples to other agonists (such as PE) can then be best displayed. The response to KCl you might think of as an "internal standard", since the individual raw contractile force of an individual sliced aortal ring would be dependent on factors such as its wall thickness and length, which would be difficult to control. Therefore, you may simply prepare the samples as permitted by the total tissue vessel lengths, and calibrate their contractility with the KCl treatment, thus avoiding variance in the data arising from sample dimensional differences.
I totally agree with Stanton's comments above. To help you in the experiments, I attach a file for our paper in rat vas deferens published in 2003, may you find some similar ideas within.
Very best wishes from Brasil,
Wilson
wilson Csantos i am also working organ bath, i want to know how to dissect vas deferens. Can you share some video please.
Thanks Shahnawaz! Well, unfortunately I have any video presentation of my experiments with rat vas deferens.
I found this in youtube:
https://www.youtube.com/watch?v=sH3JxN0vKmw
Maybe it helps you.
I also attach some files for your reading, specially the material and methods sections.
Regards and very best wishes from Brasil
Wilson
Dear Shahnawaz Ahmad Wani
you get the dissection of vas deferens from the following pdf
Dear Wilson C Santos please send materials and method section .
Dear Mudhir where is pdf paper. please send me in message or attach it here
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