Distortion problem in Abaqus explicit?

Dear all. I'm modeling the RC column with cyclic displacement by Abaqus-Explicit. The concrete model used Concrete Plastic Damage model. However I'm facing with a error about the excessive...

01 February 2021 7,726 1 View

In opensees is the ReinforcingSteel material sensitive to units of measure?

20 January 2021 7,043 2 View

The Joule-Thomson effect?

Hello I recently am suffering calculation of temperature change resulted from the Joule-Thomson effect when propylene gas at 35 oC at 2 bar passes through a membrane to vacuum pressure (~ 0 bar)...

23 December 2020 4,091 2 View

Please , can the concentration of the drug inside the nanocarriers or nanoparticles be determined using a UV spectrophotometer ?

Please, can the concentration of the drug inside the nanocarriers or nanoparticles be determined using a UV spectrophotometer after getting the calibration curve of the drug alone?

19 December 2020 4,634 5 View

How to increase titer of Herpesvirus, Adenovirus?

I'm working with Herpesvirus and Adenovirus. For Herpesvirus, I use Vero cell line to activate and check virus titer. For Adenovirus, I use Hela cell line. Recently, I could activate them, but...

16 December 2020 4,073 5 View

When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

Is the certificate getting from conference webinar is the same as the attendance certificate ?

Nowadays I have received a lot of invitations for attending conference online like webinar and get certificate and I am wandering about this certificate is the same for real attendence physically...

26 November 2020 5,344 4 View

What is the relation between the mechanism of adsorption and thermodynamic parameters?

I read some publications stated that the value of enthalaply change higher than 40 kJ/mol the adsorption will be chemisorption and below this value will be physisorption he negative values of...

22 November 2020 8,353 5 View

Which is more accurate TEM or XRD data analysis in the estimation the Nanosize of prepared nanoadsorbent material?

there many analyze to determine the exact size of the synthesized nanoparticles which is a more accurate TEM or XRD data analysis

05 November 2020 9,736 11 View

Beyond Beall's list, how to identify phony versus legit journals?

Make no mistake that journals indexed in reputable databases such as Scopus can be considered legit by default. Your contribution sure whets your appetite for learning which venue should...

27 October 2020 246 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View