What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Which are the specific cells instructed to constructing the "spikes" after a shot of a mRNA vaccine?

Hello, I am not a biology researcher, but I am working in intradisciplinary research and would like to know the answer to this question, which I have not being able to understand. This questions...

06 January 2021 3,845 4 View

What are the effects of phosphorus deficiency on dehydrogenase activity in soil bacteria and fungi?

I suspect that under phosphorus deficiency the electron transport chain in the plasmamembrane and mitochondria of soil bacteria and AM fungi, respectively, is disrupted as a result of lower ATP...

30 August 2020 4,659 3 View

What business models facilitate higher engagement of health tech start ups' user base so to retain them long term?

what business models are most popular among start-ups in general? There is very little research, if you can recommend some papers, that would be great!

17 March 2020 610 3 View

Can someone please provide references for the historical use of mycorrhizal fungi in agriculture?

Hello, I am interested in finding information regarding the historical use of mycorrhizal fungi in agriculture. I am having some difficulty finding this type of material. If you would be so kind...

21 November 2019 3,431 4 View

L cell WNT-3a-CM: why doesn't it work?

05 August 2019 1,264 5 View

Can I use RNA protect for RNA bacterial extraction inoculated on food matrix, and after that, dilute it?

Hi people! I would like to ask whether anyone of you have extracted RNA from bacteria on a food matrix. I have to inoculate bacteria on nuts, and after my processing tests, I need to extract RNA...

11 September 2018 7,343 2 View

Can any helper plasmids be used effieciently to create retroviruses?

If there is a significant distinction between two pairs of retroviral helper plasmids, what would cause the difference?

08 December 2017 9,562 3 View

What statistical analysis method should be used for analyzing the relationship between a nominal and ordinal variable?

The nominal variables are "working in a flextime schedule" and "working in a straight 8-hour schedule". The ordinal variables are scores from Likert-scales measuring Work-Family Balance and Family...

27 September 2017 1,877 11 View

If I want to do PCR of Cryptosporidium and Giardia to test, is it better to use a nested one?

05 June 2017 2,654 7 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View