what decides if alcohol dehydrogenase is bound to NADH or NADPH ? some enzymes contain NADPH as bound co-factor but does not show invitro dependency to NADP+, instead are active with NAD+ ?
NAD+/NADP+ means it is related to Mitochondria
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...
03 March 2021 2,297 3 View
03 March 2021 8,272 1 View
Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...
03 March 2021 2,037 3 View
Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...
03 March 2021 9,989 2 View
Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...
03 March 2021 6,317 1 View
i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).
03 March 2021 7,100 1 View
03 March 2021 5,360 2 View
The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...
03 March 2021 4,107 4 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...
27 February 2021 2,609 3 View
I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption
08 February 2021 7,493 3 View
The usual way of properly removing and collecting carbon nanotubes (CNT) is to sonicate the stainless steel plates coated with CNT under isopropyl alcohol (IPA) solution, remove the plates after...
28 January 2021 9,455 1 View
Hello all, we'd like to pick your brain . The enzyme we're testing requires NADPH to work. We have controls showing that our reaction needs our enzyme and our enzyme definitely needs NADPH. Since...
20 January 2021 232 2 View
I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture. I got poked after sprayed them with 70 % alcohol and dried them right away. Is...
07 January 2021 9,412 2 View
I'm not sure the steps to treat wastewater containing mixture of alcohol solvent, oils and glycerol. do we neet to separate before further treatment.
27 December 2020 6,058 6 View
In discussion with some farmers and colleagues, I came to know that poultry birds are fed alcohols to enhance their production/performance and treat the respiratory infecions. Is there any...
13 December 2020 4,700 17 View
I'm going to perform RNA-sequencing of the zebrafish brain with three different treatments, which are naive fish, alcohol withdrawal fish injected with Saline, alcohol withdrawal fish injected...
08 December 2020 4,643 2 View
In the preparation of a Lactophenol Cotton Blue, can phenol be substituted by benzul alcohol? (Question for some students in a laboratory lacking some reagents.)
03 December 2020 8,621 1 View
I am working on PANi/PVA self-standing films. For that I have dispersed PANi source i.e. emeraldine salt dispersed in NMP and ultrasonicated for few hours and the centrifuged to settle down the...
11 November 2020 831 3 View