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Questions related from Alberta liesbeth Paul
Does anybody have experience in complete lost of AF647 signal in multicolor flow? any biochemical explanation? I use a 3 laser (blue; red and violet (488; 635; 405 nm) In particular I am talking...
31 March 2019 8,126 0 View
We want to phenotype all leucocyte populations in human tonsils by multi-color flow cytometry. Do we need an enzymatic treatment for getting out all the leucocytes (DCs, Plasmacells etc.)? If so...
07 September 2018 6,374 2 View
I want to determine expression on different Human PBMC populations, cannot find it through my search on the internet. I only found a polyclonal non-conjugated anti-human ST2 from R&D. Or does...
30 October 2017 2,771 5 View
We will assess viral-antigen function in PBMCs after virus challenge. There is discussion of adding human AB serum instead of FBS or autologous serum. Any comments, taking in mind factors in...
29 June 2017 5,148 2 View
We want to change from using Leucosep tube with Ficoll to Sepmate tubes from Stemcell technologies with Lymphoprep which would save us time of isolation. Any comments on differences in recovery,...
29 June 2017 7,219 2 View
I need a positive control for my Bcl-6 staining and human Peripheral Blood CXCR5+ T helper cells are negative for this marker according to the literature. I need to make sure the staining works...
10 May 2017 9,168 0 View
We want to stain Human T cells for Notch receptors but have not found any articles showing positivety by Flow.
21 April 2017 8,704 4 View
Other cytokines or neutralizing antibodies to cytokines needed
31 March 2017 2,647 0 View
I am wondering whether to isolate PBMCs or do the staining on whole human blood, what are the advantages and disadvantages and pittfalls of whole blood staining. I will stain for gamma delta T...
16 December 2014 1,403 7 View
I wonder whether expression of certain hox-genes determines the development of tissue/organs-specificly regulatory T cells and/or Foxp3gene expression This would probably relate to retinoic acid...
11 December 2014 8,845 1 View
There are no articles describing intensive research in this field except one that mentions that only memory (mouse) T cells suppress NLRP3 activation, but not Tregs. Does anybody know some more?...
30 April 2014 3,680 0 View
I have used this method before with mice T cells for detecting cytokines and added Bref.A immediately after PMA/Ionomyc. but in the literature there is a lot of differences in the time of...
07 February 2014 8,712 23 View
I want to investigate cytokines produced by human gamma delta T cells by flow cytometry in PBMCs after isolation by microbeads. My stimulus would be PMA and ionomycine followed by inhibition by...
22 May 2013 7,258 3 View
I have a PBMC culture of 1 week with House dust mite. Besides CD4 T cells (my focus) I see that some CD3-CD4-CD8- population have also divided (as determined by Cell Trace Violet dilution)...
01 January 1970 3,573 1 View
